Effect of Auxin on Somatic Embryogenesis from Leaf Callus in Grape (Vitis spp.).
نویسندگان
چکیده
منابع مشابه
Callus Induction and Somatic Embryogenesis in Muscadine and Seedless Bunch Grapes (vitis) from Immature Ovule Culture
Pre-embryogenic calli were obtained from the culture of fertilized immature ovules of muscadine ( Vitis rotundifolia Michx.) and seedless bunch grapes ( Vitis vinifera L.) on: 1) Murashige & Turcker (MT, 1969) basal medium supplemented with 4.4 μM 2, 4-D (2, 4-dichlorophenoxyacetic acid) and 1 μM BA (6-benzyladenine) with 6.0% (w/v) sucrose; 2) Chee and Pool (CP, 1978) basal medium supplemented...
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Callus was obtained from leaf explants of Cephaelis ipecacuanha on Murashige and Skoog (MS) basal medium supplemented with 0.5 mg/l Kin, 4.0 mg/l 2,4-D and 3% (w/v) sucrose [Murashige, T., Skoog, F., 1962. Plant Physiol. 15, 437±497]. Plantlet regeneration through somatic embryogenesis was achieved on MS medium containing 2.5 mg/l Kin, 1.0 mg/l 2,4-D and 3% (w/v) sucrose. Maturation and germina...
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Induction of somatic embryogenesis in absence of exogenous auxin can clarify some basic concepts of somatic embryogenesis. Explants from leaf and petiole of Cucumber (Cucumis sativus) were used in this experiment for somatic embryogenesis using three different modified B5 basal media; boron and 2,4-D but no IAA as control (I), boron and IAA but no 2,4-D (II) and no boron, 2,4-D and IAA (III). ...
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To explore the potential for in vitro rapid regeneration of Fagonia indica Burm., cotyledon explants were obtained from 10-day old aseptically germinated seedlings that cultured on MS medium which supplemented with 1.0-5.0 µM 2,4-D. Callus was initiated from cotyledon explant on MS medium supplemented in all concentrations of 2,4-D investigated. 2,4-D was more effective than IAA and NAA for cal...
متن کاملstudy of callus induction and somatic embryogenesis in cotton
optimization of regeneration methods in cotton is necessary to improve and successful production of transgenic cotton cultivars by tissue culture technique. this study was conducted to determine the genotypic effect on callus induction in cotton using 3 genotypes (coker349, 4-s-4 and c1211) and coker 312 selected and a study was carried out in cotton research institute of gorgan using a factori...
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ژورنال
عنوان ژورنال: Japanese Journal of Breeding
سال: 1992
ISSN: 0536-3683,2185-291X
DOI: 10.1270/jsbbs1951.42.879